Metatranscriptome of Geodia parva juvenile from Northern mount - SRA

SRX4099115: Metatranscriptome of Geodia parva juvenile from Northern mount
1 ILLUMINA (Illumina HiSeq 4000) run: 55.3M spots, 16.7G bases, 6.2Gb downloads

Design: RNA was extracted with QIAGEN AllPrep DNA/RNA Mini Kit including on-column DNase treatment with the QIAGEN RNase-free DNase Set. RNA was treated with SUPERase In, quantity and quality were assessed by NanoDrop. Two technical replicates were pooled and cleaned with the QIAGEN MinElute Kit. DNA contamination was assessed by PCRs and RNA was treated with the Ambion DNA-free DNase treatment & removal kit. RNA quality was assessed by Experion, eukaryotic mRNA was removed with the Dynabeads mRNA DIRECT purification kit using the supernatant for further steps, cleaned with QIAGEN MinElute kit, quantity measured by Qubit assay, rRNA was removed with Illumina Ribo-Zero Epidemiology Kit and RNA was cleaned with QIAGEN MinElute kit.

Submitted by: GEOMAR Helmholtz Centre for Ocean Research Kiel

Study: Langseth Ridge Deep Sea Sponge Microbiome

PRJNA454581 • SRP144767 • All experiments • All runs

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This dataset was collected during expedition PS101 of research vessel POLARSTERN to the Arctic Ocean in 2016. It includes amplicon raw sequence reads (V3V4 region 16S rRNA gene survey), metagenomes and metatranscriptomes of deep-sea sponge-associated and seawater microbes.

Sample:

SAMN09064976 • SRS3265695 • All experiments • All runs

Organism: sponge metagenome

Library:

Name: PS101/0200-4A-2

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: METATRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 55.3M spots, 16.7G bases, 6.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR7182306	55,284,563	16.7G	6.2Gb	2019-06-17

ID:: 5584676

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